• Biotinylated Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-CD3 Antibody, mFc Tag at 5 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0. 14 Mu g/ml determined by ELISA.

Human CD3E&CD3D/CD3 epsilon&CD3 delta protein (Recombinant) (C-His) {Biotin} (STJP001700)

SKU:
STJP001700

£696.50 - £2,171.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human CD3E&CD3D/CD3 epsilon&CD3 delta-C-His protein was developed from hek293 and has a target region of C-His. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: CD3E
Gene ID: 916
Uniprot ID: CD3E_HUMAN
Immunogen Region: Asp23-Glu120 (CD3E) & Phe22-Ala105 (CD3D)
Immunogen: DNA sequence encoding Human CD3E&CD3D/CD3 epsilon&CD3 delta (Primary Amine Labeling) including a C-His tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.
Protein Name T-Cell Surface Glycoprotein Cd3 Epsilon Chain
T-Cell Surface Antigen T3/Leu-4 Epsilon Chain
Cd Antigen Cd3e
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-389948
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names T-Cell Surface Glycoprotein Cd3 Epsilon Chain protein
T-Cell Surface Antigen T3/Leu-4 Epsilon Chain protein
Cd Antigen Cd3e protein
CD3E protein
T3E protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance