Human Cathepsin B protein (Recombinant) (C-His) (STJP002444)
SPECIFICATIONS
HostHEK293 Cells
ConjugationUnconjugated
ImmunogenRecombinant Human Cathepsin B Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Arg18-Ile339) of human Cathepsin B (Accession #NP_001899.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Cathepsin B-C-His protein was developed in hek293 cells using the region Arg18-Ile339. For use in research applications. |
| Host | HEK293 Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008). The specific activity is >3468 pmoles/min/Mu g. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | CTSB |
| Gene ID | 1508 |
| Uniprot ID | CATB_HUMAN |
| Immunogen | Recombinant Human Cathepsin B Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Arg18-Ile339) of human Cathepsin B (Accession #NP_001899.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Arg18-Ile339 |
| Immunogen Sequence | RSRPSFHPLSDELVNYVNKR NTTWQAGHNFYNVDMSYLKR LCGTFLGGPKPPQRVMFTED LKLPASFDAREQWPQCPTIK EIRDQGSCGSCWAFGAVEAI SDRICIHTNAHVSVEVSAED LLTCCGSMCGDGCNGGYPAE AWNFWTRKGLVSGGLYESHV GCRPYSIPPCEHHVNGSRPP CTGEGDTPKCSKICEPGYSP TYKQDKHYGYNSYSVSNSEK DIMAEIYKNGPVEGAFSVY |
Additional Info
| Function | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis. |
| Protein Name | Cathepsin BApp SecretaseAppsCathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1679131Reactome: R-HSA-2022090Reactome: R-HSA-2132295Reactome: R-HSA-6798695 |
| Cellular Localisation | LysosomeMelanosomeSecretedExtracellular SpaceApical Cell MembranePeripheral Membrane ProteinExtracellular SideIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocalizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells |
| Alternative Protein Names | Cathepsin B proteinApp Secretase proteinApps proteinCathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain proteinCTSB proteinCPSB protein |
Information sourced from Uniprot.org