| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description : | This CALR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | CALR |
| Gene ID: | 811 |
| Uniprot ID: | CALR_HUMAN |
| Specificity: | This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Protein Name | Calreticulin Crp55 Calregulin Endoplasmic Reticulum Resident Protein 60 Erp60 Hacbp Grp60 |
| Database Links | Reactome: R-HSA-1236974 Reactome: R-HSA-168316 Reactome: R-HSA-3000480 Reactome: R-HSA-3000484 Reactome: R-HSA-381183 Reactome: R-HSA-901042 Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic Reticulum Lumen Cytoplasm Cytosol Secreted Extracellular Space Extracellular Matrix Cell Surface Sarcoplasmic Reticulum Lumen Cytoplasmic Vesicle Secretory Vesicle Cortical Granule Cytolytic Granule Also Found In Cell Surface (T Cells) Cytosol And Extracellular Matrix During Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical Granules In Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The Cortex In Cortical Granules Of Non-Activated Oocytes Is Exocytosed During The Cortical Reaction In Response To Oocyte Activation |
| Alternative ELISA Names | Calreticulin ELISA kit Crp55 ELISA kit Calregulin ELISA kit Endoplasmic Reticulum Resident Protein 60 ELISA kit Erp60 ELISA kit Hacbp ELISA kit Grp60 ELISA kit CALR ELISA kit CRTC ELISA kit |
| Specificity | This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-105 | 99 |
| EDTA plasma (n=8) | 86-98 | 91 |
| Cell culture media (n=8) | 90-105 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.53 | 1.36 | 4.94 | 0.52 | 1.31 | 4.68 |
| Standard deviation | 0.03 | 0.06 | 0.26 | 0.03 | 0.06 | 0.16 |
| CV (%) | 5.66 | 4.41 | 5.26 | 5.77 | 4.58 | 3.42 |