| Post Translational Modifications | Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction. Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1. |
| Function | Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Promotes the centromeric localization of TOP2A. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. |
| Protein Name | Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1Hbub1Bub1a |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreNuclear In Interphase CellsAccumulates Gradually During G1 And S Phase Of The Cell CyclePeaks At G2/MAnd Drops Dramatically After MitosisLocalizes To The Outer KinetochoreKinetochore Localization Is Required For Normal Mitotic Timing And Checkpoint Response To Spindle Damage And Occurs Very Early In ProphaseAurkbKnl1 And Incenp Are Required For Kinetochore Localization |
| Alternative Protein Names | Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1 proteinHbub1 proteinBub1a proteinBUB1 proteinBUB1L protein |
Information sourced from Uniprot.org
