| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant human BTLA at 3 Mu g/mL (100 Mu L/well) can bind Biotinylated Recombinant human HVEM with a linear range of 18-72 ng/mL. |
| Gene Symbol: | BTLA |
| Gene ID: | 151888 |
| Uniprot ID: | BTLA_HUMAN |
| Immunogen Region: | Lys31-Thr134 |
| Immunogen: | Active Recombinant Human BTLA Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Lys31-Thr134) of human BTLA (Accession #NP_001078826.1.) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Sequence: | KESCDVQLYIKRQSEHSILA GDPFELECPVKYCANRPHVT WCKLNGTTCVKLEDRQTSWK EEKNISFFILHFEPVLPNDN GSYRCSANFQSNLIESHSTT LYVT |
| Post Translational Modifications | Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11. N-glycosylated. |
| Function | Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells. |
| Protein Name | B- And T-Lymphocyte AttenuatorB- And T-Lymphocyte-Associated ProteinCd Antigen Cd272 |
| Database Links | Reactome: R-HSA-388841 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | B- And T-Lymphocyte Attenuator proteinB- And T-Lymphocyte-Associated Protein proteinCd Antigen Cd272 proteinBTLA protein |
Information sourced from Uniprot.org