| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human BRAF-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | BRAF |
| Gene ID: | 673 |
| Uniprot ID: | BRAF_HUMAN |
| Immunogen Region: | Asn500-His766 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1. Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC).this relieves inactivation and stimulates kinase activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
| Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. |
| Protein Name | Serine/Threonine-Protein Kinase B-RafProto-Oncogene B-RafP94V-Raf Murine Sarcoma Viral Oncogene Homolog B1 |
| Database Links | Reactome: R-HSA-1295596Reactome: R-HSA-170968Reactome: R-HSA-170984Reactome: R-HSA-187706Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9726840Reactome: R-HSA-9726842 |
| Cellular Localisation | NucleusCytoplasmCell MembraneColocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes |
| Alternative Protein Names | Serine/Threonine-Protein Kinase B-Raf proteinProto-Oncogene B-Raf proteinP94 proteinV-Raf Murine Sarcoma Viral Oncogene Homolog B1 proteinBRAF proteinBRAF1 proteinRAFB1 protein |
Information sourced from Uniprot.org
