Human BMP15 (Bone morphogenetic protein 15) Sandwich ELISA Kit (STJE0004057)

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STJE0004057
£163.50 - £357.50
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description : This BMP15 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: BMP15
Gene ID: 9210
Uniprot ID: BMP15_HUMAN
Specificity: This kit recognizes Human BMP-15 in samples. No significant cross-reactivity or interference between Human BMP-15 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Bone Morphogenetic Protein 15
Bmp-15
Growth/Differentiation Factor 9b
Gdf-9b
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative ELISA Names Bone Morphogenetic Protein 15 ELISA kit
Bmp-15 ELISA kit
Growth/Differentiation Factor 9b ELISA kit
Gdf-9b ELISA kit
BMP15 ELISA kit
GDF9B ELISA kit
Specificity This kit recognizes Human BMP-15 in samples. No significant cross-reactivity or interference between Human BMP-15 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-110 102
EDTA plasma (n=8) 96-108 103
Cell culture media (n=8) 86-95 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 44.05 110.35 434.98 42.77 111.81 466.66
Standard deviation 2.58 5.27 19.27 2.19 5.33 20.67
CV (%) 5.86 4.78 4.43 5.12 4.77 4.43

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