| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.107ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description : | This BIRC6 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | BIRC6 |
| Gene ID: | 57448 |
| Uniprot ID: | BIRC6_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in brain cancer cells. |
| Post Translational Modifications | Ubiquitinated.mediated by RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis. Deubiquitinated by USP8/UBPY. Autoubiquitinated.mediated by E1 ubiquitin activating enzyme UBA6. Proteolytically cleaved. Acts as substrate for CASP3, CASP6, CASP7, CASP9 and HTRA2. |
| Function | Anti-apoptotic protein known as inhibitor of apoptosis (IAP) which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Unlike most IAPs, does not contain a RING domain and it is not a RING-type E3 ligase. Instead acts as a dual E2/E3 enzyme that combines ubiquitin conjugating (E2) and ubiquitin ligase (E3) activities in a single polypeptide. Ubiquitination is mediated by a non-canonical E1 ubiquitin activating enzyme UBA6. Ubiquitinates CASP3, CASP7 and CASP9 and inhibits their caspase activity.also ubiquitinates their procaspases but to a weaker extent. Ubiquitinates pro-apoptotic factors DIABLO/SMAC and HTRA2. DIABLO/SMAC antagonizes the caspase inhibition activity of BIRC6 by competing for the same binding sites as the caspases. Ubiquitinates the autophagy protein MAP1LC3B.this activity is also inhibited by DIABLO/SMAC. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. |
| Protein Name | Dual E2 Ubiquitin-Conjugating Enzyme/E3 Ubiquitin-Protein Ligase Birc6 Bir Repeat-Containing Ubiquitin-Conjugating Enzyme Bruce Baculoviral Iap Repeat-Containing Protein 6 Ubiquitin-Conjugating Bir Domain Enzyme Apollon Apollon |
| Database Links | Reactome: R-HSA-9700645 Reactome: R-HSA-9725370 |
| Cellular Localisation | Golgi Apparatus Trans-Golgi Network Membrane Endosome Cytoplasm Cytoskeleton Spindle Pole Microtubule Organizing Center Centrosome Midbody Midbody Ring Exhibits Cell Cycle-Dependent Localization Concentrates In A Pericentriolar Compartment In Interphase Moves Partially To Spindle Poles In Metaphase And Finally Localizes To The Spindle Midzone And The Midbody In Telophase And During Cytokinesis On The Midbody Localizes To The Midbody Ring Also Called Flemming Body In Interphase Cells Localizes To The Trans-Golgi Network Membrane And Endosomes During Cytokinesis A Fraction Moves To The Midzone Where It Specifically Arrives At The Midbody Ring After Abscission Completion Travels With The Midbody Remnant Into One Daughter Cell And Remains Bound To It Until A New Midbody Ring Is Formed During The Next Cell Division |
| Alternative ELISA Names | Dual E2 Ubiquitin-Conjugating Enzyme/E3 Ubiquitin-Protein Ligase Birc6 ELISA kit Bir Repeat-Containing Ubiquitin-Conjugating Enzyme ELISA kit Bruce ELISA kit Baculoviral Iap Repeat-Containing Protein 6 ELISA kit Ubiquitin-Conjugating Bir Domain Enzyme Apollon ELISA kit Apollon ELISA kit BIRC6 ELISA kit KIAA1289 ELISA kit |
| output |
Information sourced from Uniprot.org