| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.107ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This BIRC6 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
BIRC6 |
| Gene ID: |
57448 |
| Uniprot ID: |
BIRC6_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in brain cancer cells. |
| Post Translational Modifications | Ubiquitinated.mediated by RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis. Deubiquitinated by USP8/UBPY. Autoubiquitinated.mediated by E1 ubiquitin activating enzyme UBA6. Proteolytically cleaved. Acts as substrate for CASP3, CASP6, CASP7, CASP9 and HTRA2. |
| Function | Anti-apoptotic protein known as inhibitor of apoptosis (IAP) which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Unlike most IAPs, does not contain a RING domain and it is not a RING-type E3 ligase. Instead acts as a dual E2/E3 enzyme that combines ubiquitin conjugating (E2) and ubiquitin ligase (E3) activities in a single polypeptide. Ubiquitination is mediated by a non-canonical E1 ubiquitin activating enzyme UBA6. Ubiquitinates CASP3, CASP7 and CASP9 and inhibits their caspase activity.also ubiquitinates their procaspases but to a weaker extent. Ubiquitinates pro-apoptotic factors DIABLO/SMAC and HTRA2. DIABLO/SMAC antagonizes the caspase inhibition activity of BIRC6 by competing for the same binding sites as the caspases. Ubiquitinates the autophagy protein MAP1LC3B.this activity is also inhibited by DIABLO/SMAC. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. |
| Protein Name | Dual E2 Ubiquitin-Conjugating Enzyme/E3 Ubiquitin-Protein Ligase Birc6Bir Repeat-Containing Ubiquitin-Conjugating EnzymeBruceBaculoviral Iap Repeat-Containing Protein 6Ubiquitin-Conjugating Bir Domain Enzyme ApollonApollon |
| Database Links | Reactome: R-HSA-9700645Reactome: R-HSA-9725370 |
| Cellular Localisation | Golgi ApparatusTrans-Golgi Network MembraneEndosomeCytoplasmCytoskeletonSpindle PoleMicrotubule Organizing CenterCentrosomeMidbodyMidbody RingExhibits Cell Cycle-Dependent LocalizationConcentrates In A Pericentriolar Compartment In InterphaseMoves Partially To Spindle Poles In MetaphaseAnd Finally Localizes To The Spindle Midzone And The Midbody In Telophase And During CytokinesisOn The MidbodyLocalizes To The Midbody RingAlso Called Flemming BodyIn Interphase CellsLocalizes To The Trans-Golgi Network Membrane And EndosomesDuring CytokinesisA Fraction Moves To The Midzone Where It Specifically Arrives At The Midbody RingAfter Abscission CompletionTravels With The Midbody Remnant Into One Daughter CellAnd Remains Bound To It Until A New Midbody Ring Is Formed During The Next Cell Division |
| Alternative ELISA Names | Dual E2 Ubiquitin-Conjugating Enzyme/E3 Ubiquitin-Protein Ligase Birc6 ELISA kitBir Repeat-Containing Ubiquitin-Conjugating Enzyme ELISA kitBruce ELISA kitBaculoviral Iap Repeat-Containing Protein 6 ELISA kitUbiquitin-Conjugating Bir Domain Enzyme Apollon ELISA kitApollon ELISA kitBIRC6 ELISA kitKIAA1289 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
