Human Beta-2-microglobulin/B2M protein (Recombinant) (C-His) (STJP000881)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Beta-2-microglobulin/B2M Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ile21-Met119) of human B2M/Beta-2-microglobulin (Accession #NP_004039.1) fused with a 6×His tag at the C-termin
General Information
| Short Description | Recombinant-Human Beta-2-microglobulin/B2M-C-His protein was developed in hek293 cells using the region Ile21-Met119. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B2M at 2 Mu g/mL (100 Mu L/well) can bind Recombinant Human CD8 alpha with a linear range of 31.25-341 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | B2M |
| Gene ID | 567 |
| Uniprot ID | B2MG_HUMAN |
| Immunogen | Recombinant Human Beta-2-microglobulin/B2M Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ile21-Met119) of human B2M/Beta-2-microglobulin (Accession #NP_004039.1) fused with a 6×His tag at the C-termin |
| Immunogen Region | Ile21-Met119 |
| Immunogen Sequence | IQRTPKIQVYSRHPAENGKS NFLNCYVSGFHPSDIEVDLL KNGERIEKVEHSDLSFSKDW SFYLLYYTEFTPTEKDEYAC RVNHVTLSQPKIVKWDRDM |
Additional Info
| Post Translational Modifications | Glycation of Ile-21 is observed in long-term hemodialysis patients. |
| Function | Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation. |
| Protein Name | Beta-2-Microglobulin Cleaved Into - Beta-2-Microglobulin Form Pi 5.3 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-1236977Reactome: R-HSA-164940Reactome: R-HSA-198933Reactome: R-HSA-2172127Reactome: R-HSA-2424491Reactome: R-HSA-6798695Reactome: R-HSA-877300Reactome: R-HSA-9637628Reactome: R-HSA-9705671Reactome: R-HSA-977225Reactome: R-HSA-983170 |
| Cellular Localisation | SecretedCell SurfaceDetected In Serum And Urine(Microbial Infection) In The Presence Of MTuberculosis Esxa-Esxb Complex Decreased Amounts Of B2m Are Found On The Cell Surface |
| Alternative Protein Names | Beta-2-Microglobulin Cleaved Into - Beta-2-Microglobulin Form Pi 5.3 proteinB2M proteinCDABP0092 proteinHDCMA22P protein |
Information sourced from Uniprot.org