• Biotinylated Human B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • Immobilized Biotinylated Human B7-2, His Tag at 0. 5 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0. 2 Mu g/ml determined by ELISA.

Human B7-2/CD86 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001765)

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STJP001765

£618.50 - £1,909.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human B7-2/CD86-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: CD86
Gene ID: 942
Uniprot ID: CD86_HUMAN
Immunogen Region: Leu26-Pro247
Immunogen: DNA sequence encoding Human B7-2/CD86 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then test biotinylated site specific using the AVItag biotinylation technology.test NA
Post Translational Modifications Polyubiquitinated.which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. Isoform 2: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Protein Name T-Lymphocyte Activation Antigen Cd86
Activation B7-2 Antigen
B70
Bu63
Ctla-4 Counter-Receptor B7.2
Fun-1
Cd Antigen Cd86
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-389356
Reactome: R-HSA-389357
Reactome: R-HSA-389359
Reactome: R-HSA-389513
Reactome: R-HSA-6783783
Reactome: R-HSA-6811558
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names T-Lymphocyte Activation Antigen Cd86 protein
Activation B7-2 Antigen protein
B70 protein
Bu63 protein
Ctla-4 Counter-Receptor B7.2 protein
Fun-1 protein
Cd Antigen Cd86 protein
CD86 protein
CD28LG2 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance