Human B7-1/CD80 protein (Recombinant) (C-His&Avi) {Biotin} (STJP020000)
SPECIFICATIONS
HostHEK293 cells
ConjugationBiotin
ImmunogenBiotinylated Recombinant Human B7-1/CD80 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val35-Asn242) of Human B7-1/CD80 (Accession #P33681-1) fused with a C-His&Avi tag at the C-terminus.
General Information
| Short Description | Recombinant-Human B7-1/CD80-C-His&Avi protein was developed in hek293 cells using the region Val35-Asn242. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CD80 |
| Gene ID | 941 |
| Uniprot ID | CD80_HUMAN |
| Immunogen | Biotinylated Recombinant Human B7-1/CD80 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val35-Asn242) of Human B7-1/CD80 (Accession #P33681-1) fused with a C-His&Avi tag at the C-terminus. |
| Immunogen Region | Val35-Asn242 |
| Immunogen Sequence | VIHVTKEVKEVATLSCGHNV SVEELAQTRIYWQKEKKMVL TMMSGDMNIWPEYKNRTIFD ITNNLSIVILALRPSDEGTY ECVVLKYEKDAFKREHLAEV TLSVKADFPTPSISDFEIPT SNIRRIICSTSGGFPEPHLS WLENGEELNAINTTVSQDPE TELYAVSSKLDFNMTTNHSF MCLIKYGHLRVNQTFNWNTT KQEHFPDN |
Additional Info
| Tissue Specificity | Expressed on activated B-cells, macrophages and dendritic cells. |
| Post Translational Modifications | Palmitoylated by ZDHHC20.palmitoylation protects CD80 from ubiquitin-mediated degradation, regulating the protein stability, and ensures its accurate plasma membrane localization. |
| Function | Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells. In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Also acts as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses. (Microbial infection) Acts as a receptor for adenovirus subgroup B. |
| Protein Name | T-Lymphocyte Activation Antigen Cd80Activation B7-1 AntigenBb1Ctla-4 Counter-Receptor B7.1B7Cd Antigen Cd80 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-2219530Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-389513Reactome: R-HSA-6783783Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | T-Lymphocyte Activation Antigen Cd80 proteinActivation B7-1 Antigen proteinBb1 proteinCtla-4 Counter-Receptor B7.1 proteinB7 proteinCd Antigen Cd80 proteinCD80 proteinCD28LG proteinCD28LG1 proteinLAB7 protein |
Information sourced from Uniprot.org