Human APOH (Beta-2-glycoprotein 1) CLIA Kit (STJC0000278)

Human APOH (Beta-2-glycoprotein 1) CLIA Kit (STJC0000278)

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STJC0000278
£180.50 - £462.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description : This human APOH kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: APOH
Gene ID: 350
Uniprot ID: APOH_HUMAN
Specificity: This kit recognizes Human ApoH in samples. No significant cross-reactivity or interference between Human ApoH and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed by the liver and secreted in plasma.
Post Translational Modifications N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele.
Function Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
Protein Name Beta-2-Glycoprotein 1
Apc Inhibitor
Activated Protein C-Binding Protein
Anticardiolipin Cofactor
Apolipoprotein H
Apo-H
Beta-2-Glycoprotein I
B2gpi
Beta(2gpi
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Alternative CLIA Names Beta-2-Glycoprotein 1 CLIA kit
Apc Inhibitor CLIA kit
Activated Protein C-Binding Protein CLIA kit
Anticardiolipin Cofactor CLIA kit
Apolipoprotein H CLIA kit
Apo-H CLIA kit
Beta-2-Glycoprotein I CLIA kit
B2gpi CLIA kit
Beta(2gpi CLIA kit
APOH CLIA kit
B2G1 CLIA kit
Specificity This kit recognizes Human ApoH in samples. No significant cross-reactivity or interference between Human ApoH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 94
EDTA plasma(n=8) 88-100 93
Cell culture media(n=8) 94-111 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 247.79 574.87 1815.52 242.73 613.72 1782.40
Standard deviation 30.08 59.44 204.79 26.34 45.11 159.35
CV (%) 12.14 10.34 11.28 10.85 7.35 8.94

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