Human ANGPT4 (Angiopoietin-4) CLIA Kit (STJC0000200)

Human ANGPT4 (Angiopoietin-4) CLIA Kit (STJC0000200)

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STJC0000200
£180.50 - £462.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description : This human ANGPT4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: ANGPT4
Gene ID: 51378
Uniprot ID: ANGP4_HUMAN
Specificity: This kit recognizes Human ANGPT4 in samples. No significant cross-reactivity or interference between Human ANGPT4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Highly expressed in the lung with much lower levels found in other tissues.
Function Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.
Protein Name Angiopoietin-4
Ang-4
Angiopoietin-3
Ang-3
Database Links Reactome: R-HSA-210993
Cellular Localisation Secreted
Alternative CLIA Names Angiopoietin-4 CLIA kit
Ang-4 CLIA kit
Angiopoietin-3 CLIA kit
Ang-3 CLIA kit
ANGPT4 CLIA kit
ANG3 CLIA kit
ANG4 CLIA kit
Specificity This kit recognizes Human ANGPT4 in samples. No significant cross-reactivity or interference between Human ANGPT4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-112 104
EDTA plasma(n=8) 89-101 94
Cell culture media(n=8) 100-116 106

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 102.86 300.31 973.57 99.81 291.76 1051.18
Standard deviation 11.51 28.50 62.60 11.26 23.31 87.88
CV (%) 11.19 9.49 6.43 11.28 7.99 8.36

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