Human ANG1 (Angiopoietin-1) Antibody Pair (STJAP00119)
General Information
| Short Description | This ANG1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | ANGPT1 |
| Gene ID | 284 |
| Uniprot ID | ANGP1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human ANG1 protein.Detection Immunogen: Recombinant Human ANG1 protein. |
Additional Info
| Function | Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. |
| Protein Name | Angiopoietin-1Ang-1 |
| Database Links | Reactome: R-HSA-210993Reactome: R-HSA-5673001 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Angiopoietin-1 antibody pairAng-1 antibody pairANGPT1 antibody pairKIAA0003 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human ANG1 (Angiopoietin-1) Antibody Pair (STJAP00119)
This antibody pair detects human Angiopoietin-1 (ANG1, UniProt Q15389) and is validated for reactivity against human samples only.
The capture antibody is a mouse monoclonal antibody supplied unconjugated, while the detection antibody is a goat polyclonal antibody supplied conjugated to biotin. Both are provided at a concentration of 0.5 mg/mL.
This pair has been validated for use in ELISA. No other assay formats are listed for this product.
Both the capture and detection antibodies were raised against recombinant human ANG1 protein. The capture antibody was purified by Protein A or G, and the detection antibody was purified by antigen affinity.
Both the capture and detection antibodies are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4. The components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.