| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Human AFP at 2 Mu g/mL (100 Mu L/well) can bind AFP Mouse mAb with a linear range of 0.061-5.431 ng/mL. |
| Gene Symbol: | AFP |
| Gene ID: | 174 |
| Uniprot ID: | FETA_HUMAN |
| Immunogen: | Recombinant Human Alpha-fetoprotein/AFP Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg19-Val609) of human Alpha-fetoprotein (Accession #NP_001125.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | RTLHRNEYGIASILDSYQCT AEISLADLATIFFAQFVQEA TYKEVSKMVKDALTAIEKPT GDEQSSGCLENQLPAFLEEL CHEKEILEKYGHSDCCSQSE EGRHNCFLAHKKPTPASIPL FQVPEPVTSCEAYEEDRETF MNKFIYEIARRHPFLYAPTI LLWAARYDKIIPSCCKAENA VECFQTKAATVTKELRESSL LNQHACAVMKNFGTRTFQAI TVTKLSQKFTKVNFTEIQK |
| Tissue Specificity | Plasma. Synthesized by the fetal liver and yolk sac. |
| Post Translational Modifications | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. |
| Protein Name | Alpha-FetoproteinAlpha-1-FetoproteinAlpha-Fetoglobulin |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Alpha-FetoproteinAlpha-1-FetoproteinAlpha-Fetoglobulin proteinAFP proteinHPAFP protein |
Information sourced from Uniprot.org
