• Biotinylated Human ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human ALCAM is greater than 95% as determined by SEC-HPLC.
  • Immobilized Biotinylated Human ALCAM, His Tag at 2 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0. 48 Mu g/ml determined by ELISA.

Human ALCAM/CD166 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001564)

SKU:
STJP001564

£618.50 - £1,909.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human ALCAM/CD166-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: ALCAM
Gene ID: 214
Uniprot ID: CD166_HUMAN
Immunogen Region: Trp28-Ala526
Immunogen: DNA sequence encoding Human ALCAM/CD166 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Glycosylated.
Function Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation. Required for normal hematopoietic stem cell engraftment in the bone marrow. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis.required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation. Promotes neurite extension, axon growth and axon guidance.axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons. Isoform 3: Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.
Protein Name Cd166 Antigen
Activated Leukocyte Cell Adhesion Molecule
Cd Antigen Cd166
Database Links Reactome: R-HSA-373760
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Axon
Dendrite
Detected At The Immunological Synapse
I
E
At The Contact Zone Between Antigen-Presenting Dendritic Cells And T-Cells
Colocalizes With Cd6 And The Tcr/Cd3 Complex At The Immunological Synapse
Isoform 3: Secreted
Alternative Protein Names Cd166 Antigen protein
Activated Leukocyte Cell Adhesion Molecule protein
Cd Antigen Cd166 protein
ALCAM protein
MEMD protein

Information sourced from Uniprot.org

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