| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human AKT3-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | AKT3 |
| Gene ID: | 10000 |
| Uniprot ID: | AKT3_HUMAN |
| Immunogen Region: | Met1-Lys143 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Phosphorylation on Thr-305 and Ser-472 is required for full activity. Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1. |
| Function | AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. |
| Protein Name | Rac-Gamma Serine/Threonine-Protein KinaseProtein Kinase Akt-3Protein Kinase B GammaPkb GammaRac-Pk-GammaStk-2 |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-1257604Reactome: R-HSA-1358803Reactome: R-HSA-198323Reactome: R-HSA-198693Reactome: R-HSA-199418Reactome: R-HSA-211163Reactome: R-HSA-389357Reactome: R-HSA-389513Reactome: R-HSA-392451Reactome: R-HSA-5218920Reactome: R-HSA-5628897Reactome: R-HSA-5674400Reactome: R-HSA-6804757Reactome: R-HSA-6804758Reactome: R-HSA-6804759Reactome: R-HSA-69202Reactome: R-HSA-69656Reactome: R-HSA-8876198Reactome: R-HSA-8941332Reactome: R-HSA-8948751Reactome: R-HSA-9607240Reactome: R-HSA-9614399Reactome: R-HSA-9634638Reactome: R-HSA-9755511Reactome: R-HSA-9755779Reactome: R-HSA-9856649 |
| Cellular Localisation | NucleusCytoplasmMembranePeripheral Membrane ProteinMembrane-Associated After Cell Stimulation Leading To Its Translocation |
| Alternative Protein Names | Rac-Gamma Serine/Threonine-Protein Kinase proteinProtein Kinase Akt-3 proteinProtein Kinase B Gamma proteinPkb Gamma proteinRac-Pk-Gamma proteinStk-2 proteinAKT3 proteinPKBG protein |
Information sourced from Uniprot.org