| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description : | This ADH Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 2.5h |
| Detection: | Colormetric |
| Gene Symbol: | AVP |
| Gene ID: | 551 |
| Uniprot ID: | NEU2_HUMAN |
| Specificity: | This kit recognizes Human ADH in samples. No significant cross-reactivity or interference between Human ADH and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Protein Name | Vasopressin-Neurophysin 2-CopeptinAvp-Npii Cleaved Into - Arg-VasopressinArginine-Vasopressin - Neurophysin 2Neurophysin-Ii - Copeptin |
| Database Links | Reactome: R-HSA-1368108Reactome: R-HSA-388479Reactome: R-HSA-416476Reactome: R-HSA-418555Reactome: R-HSA-432040Reactome: R-HSA-5619099Reactome: R-HSA-879518Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9036092 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Vasopressin-Neurophysin 2-Copeptin ELISA kitAvp-Npii Cleaved Into - Arg-Vasopressin ELISA kitArginine-Vasopressin - Neurophysin 2 ELISA kitNeurophysin-Ii - Copeptin ELISA kitAVP ELISA kitARVP ELISA kitVP ELISA kit |
| Specificity | This kit recognizes ADH in samples. No significant cross-reactivity or interference between ADH and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-102 | 96 |
| EDTA plasma(n=8) | 92-103 | 98 |
| Cell culture media(n=8) | 91-104 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 45.80 | 140.60 | 362.30 | 49.30 | 127.00 | 335.60 |
| Standard deviation | 2.70 | 5.80 | 12.00 | 3.10 | 6.20 | 11.10 |
| CV (%) | 5.90 | 4.13 | 3.31 | 6.29 | 4.88 | 3.31 |