| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.19ng/mL |
| Detection Limit: | 0.31~20ng/mL |
| Short Description : | This ADAMTS13 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | ADAMTS13 |
| Gene ID: | 11093 |
| Uniprot ID: | ATS13_HUMAN |
| Specificity: | This kit recognizes Human VWFCP/ADAMTS13 in samples. No significant cross-reactivity or interference between Human VWFCP/ADAMTS13 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS13. May also be C-glycosylated on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and also N-glycosylated. These other glycosylations can also facilitate secretion. The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. |
| Function | Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. |
| Protein Name | A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13 Adam-Ts 13 Adam-Ts13 Adamts-13 Von Willebrand Factor-Cleaving Protease Vwf-Cp Vwf-Cleaving Protease |
| Database Links | Reactome: R-HSA-5083635 Reactome: R-HSA-5173214 Reactome: R-HSA-75892 Reactome: R-HSA-9845619 Reactome: R-HSA-9845621 |
| Cellular Localisation | Secreted Secretion Enhanced By O-Fucosylation Of Tsp Type-1 Repeats |
| Alternative ELISA Names | A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13 ELISA kit Adam-Ts 13 ELISA kit Adam-Ts13 ELISA kit Adamts-13 ELISA kit Von Willebrand Factor-Cleaving Protease ELISA kit Vwf-Cp ELISA kit Vwf-Cleaving Protease ELISA kit ADAMTS13 ELISA kit C9orf8 ELISA kit UNQ6102 ELISA kit PRO20085 ELISA kit |
| Specificity | This kit recognizes Human VWFCP/ADAMTS13 in samples. No significant cross-reactivity or interference between Human VWFCP/ADAMTS13 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 95-108 | 101 |
| EDTA plasma (n=8) | 93-106 | 100 |
| Cell culture media (n=8) | 89-102 | 95 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.99 | 1.99 | 8.23 | 0.98 | 1.84 | 8.80 |
| Standard deviation | 0.06 | 0.10 | 0.43 | 0.06 | 0.08 | 0.45 |
| CV (%) | 6.06 | 5.03 | 5.22 | 6.12 | 4.35 | 5.11 |