Name: Human ACTN2 (Alpha-actinin-2) CLIA Kit (STJC0000167)
SKU: STJC0000167
Availability: InStock

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	46.88pg/mL
Detection Limit:	78.13~5000pg/mL
Short Description :	This human ACTN2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	ACTN2
Gene ID:	88
Uniprot ID:	ACTN2_HUMAN
Specificity:	This kit recognizes Human ACTN2 in samples. No significant cross-reactivity or interference between Human ACTN2 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in both skeletal and cardiac muscle.
Post Translational Modifications	Ubiquitinated by FBXL22, leading to proteasomal degradation.
Function	F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Protein Name	Alpha-Actinin-2 Alpha-Actinin Skeletal Muscle Isoform 2 F-Actin Cross-Linking Protein
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-373753 Reactome: R-HSA-390522 Reactome: R-HSA-438066 Reactome: R-HSA-442982 Reactome: R-HSA-5673001 Reactome: R-HSA-9609736 Reactome: R-HSA-9617324 Reactome: R-HSA-9620244
Cellular Localisation	Cytoplasm Myofibril Sarcomere Z Line Colocalizes With Myoz1 And Flnc At The Z-Lines Of Skeletal Muscle
Alternative CLIA Names	Alpha-Actinin-2 CLIA kit Alpha-Actinin Skeletal Muscle Isoform 2 CLIA kit F-Actin Cross-Linking Protein CLIA kit ACTN2 CLIA kit
Specificity	This kit recognizes Human ACTN2 in samples. No significant cross-reactivity or interference between Human ACTN2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	255.15	495.46	1831.61	235.73	520.10	1693.41
Standard deviation	32.07	41.72	152.76	20.58	40.10	187.46
CV (%)	12.57	8.42	8.34	8.73	7.71	11.07

Model	Name	Reactivity	Application
STJC0000168	Human ACTN3 (Alpha-actinin-3) CLIA Kit (STJC0000168)	Human	CLIA
STJC0000480	Human SNCA (Alpha-synuclein) CLIA Kit (STJC0000480)	Human	CLIA
STJC0000749	Human ORM2 (Alpha-1-acid glycoprotein 2) CLIA Kit (STJC0000749)	Human	CLIA

Human ACTN2 (Alpha-actinin-2) CLIA Kit (STJC0000167)