Function | Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. |
Protein Name | Tartrate-Resistant Acid Phosphatase Type 5Tr-ApTartrate-Resistant Acid AtpaseTratpaseType 5 Acid Phosphatase |
Database Links | Reactome: R-HSA-196843 |
Cellular Localisation | Lysosome |
Alternative CLIA Names | Tartrate-Resistant Acid Phosphatase Type 5 CLIA kitTr-Ap CLIA kitTartrate-Resistant Acid Atpase CLIA kitTratpase CLIA kitType 5 Acid Phosphatase CLIA kitACP5 CLIA kit |
Specificity | This kit recognizes Human ACP5 in samples. No significant cross-reactivity or interference between Human ACP5 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 90-106 | 98 |
EDTA plasma(n=8) | 90-105 | 96 |
Cell culture media(n=8) | 89-102 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 0.55 | 0.91 | 4.72 | 0.56 | 0.97 | 4.61 |
Standard deviation | 0.07 | 0.10 | 0.51 | 0.06 | 0.11 | 0.39 |
CV (%) | 12.73 | 10.99 | 10.81 | 10.71 | 11.34 | 8.46 |