| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human ACE2/ACEH-C-hFc-Avi protein was developed from hek293 and has a target region of C-hFc-Avi. For use in research applications. |
| Conjugation: |
Biotin |
| Formulation: |
Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: |
Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Storage Instruction: |
The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: |
ACE2 |
| Gene ID: |
59272 |
| Uniprot ID: |
ACE2_HUMAN |
| Immunogen Region: |
Gln18-Ser740 |
| Immunogen: |
DNA sequence encoding Human ACE2/ACEH including a C-hFc-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Post Translational Modifications | N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. Phosphorylated. Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains. |
| Function | Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, Pyr1apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2: Non-functional as a carboxypeptidase. Isoform 2: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2. |
| Protein Name | Angiotensin-Converting Enzyme 2Angiotensin-Converting Enzyme HomologAcehAngiotensin-Converting Enzyme-Related CarboxypeptidaseAce-Related CarboxypeptidaseMetalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 |
| Database Links | Reactome: R-HSA-2022377Reactome: R-HSA-9678110Reactome: R-HSA-9679191Reactome: R-HSA-9694614Reactome: R-HSA-9733458 |
| Cellular Localisation | Processed Angiotensin-Converting Enzyme 2: SecretedCell MembraneSingle-Pass Type I Membrane ProteinCytoplasmCell ProjectionCiliumApical Cell MembraneDetected In Both Cell Membrane And Cytoplasm In NeuronsIsoform 2: Apical Cell Membrane |
| Alternative Protein Names | Angiotensin-Converting Enzyme 2 proteinAngiotensin-Converting Enzyme Homolog proteinAceh proteinAngiotensin-Converting Enzyme-Related Carboxypeptidase proteinAce-Related Carboxypeptidase proteinMetalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 proteinACE2 proteinUNQ868 proteinPRO1885 protein |
Information sourced from Uniprot.org
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