Human ABCA3 protein (Recombinant) (N-His) (STJP005910)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ABCA3-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | ABCA3 |
| Gene ID | 21 |
| Uniprot ID | ABCA3_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp1358-Phe1635 |
Additional Info
| Post Translational Modifications | N-glycosylated. Localization at intracellular vesicles is accompanied by processing of oligosaccharide from high mannose type to complex type. N-linked glycosylation at Asn-124 and Asn-140 is required for stability and efficient anterograde trafficking and prevents from proteasomal degradation. Proteolytically cleaved by CTSL and to a lower extent by CTSB within multivesicular bodies (MVB) and lamellar bodies (LB) leading to a mature form of 150 kDa. |
| Function | Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant. Transports preferentially phosphatidylcholine containing short acyl chains. In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity. |
| Protein Name | Phospholipid-Transporting Atpase Abca3Abc-C TransporterAtp-Binding Cassette Sub-Family A Member 3Atp-Binding Cassette Transporter 3Atp-Binding Cassette 3Xenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form |
| Database Links | Reactome: R-HSA-1369062Reactome: R-HSA-5683678Reactome: R-HSA-5683826Reactome: R-HSA-5688399 |
| Cellular Localisation | EndosomeMultivesicular Body MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneLate Endosome MembraneLysosome MembraneLocalized In The Limiting Membrane Of Lamellar Bodies In Lung Alveolar Type Ii CellsTrafficks Via The GolgiSorting Vesicles (Svs) And Late Endosome/Multivesicular Body Network Directly To The Outer Membrane Of Lamellar Bodies In At2 Lung Epithelial Cells Or To Lysosomes And Lysosomal-Related Organelles (Lros) In Other Cells Where Undergoes Proteolytic Cleavage And Oligosaccharide Processing From High Mannose Type To Complex TypeOligomers Formation Takes Place In A Post-Endoplasmic Reticulum Compartment |
| Alternative Protein Names | Phospholipid-Transporting Atpase Abca3 proteinAbc-C Transporter proteinAtp-Binding Cassette Sub-Family A Member 3 proteinAtp-Binding Cassette Transporter 3 proteinAtp-Binding Cassette 3 proteinXenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form proteinABCA3 proteinABC3 protein |
Information sourced from Uniprot.org