| Applications: | Immunodepletion/Immunocompetition |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | GTPase HRAS Blocking Peptide is synthetically produced from the 139-189 sequence and is suitable for use in western blot applications. |
| Formulation: | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | HRAS |
| Gene ID: | 3265 |
| Uniprot ID: | RASH_HUMAN |
| Immunogen Region: | 139-189 |
| Immunogen: | Synthetic peptide taken within amino acid region 139-189 on human GTPase HRAS protein. |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated.critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Fatty-acylated at Lys-170. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to inhibit Ras signaling. |
| Function | Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
| Peptide Name | Gtpase HrasH-Ras-1Ha-RasTransforming Protein P21C-H-RasP21ras Cleaved Into - Gtpase Hras - N-Terminally Processed |
| Database Links | Reactome: R-HSA-112412Reactome: R-HSA-1169092Reactome: R-HSA-1236382Reactome: R-HSA-1250196Reactome: R-HSA-1250347Reactome: R-HSA-1433557Reactome: R-HSA-167044Reactome: R-HSA-171007Reactome: R-HSA-179812Reactome: R-HSA-180336Reactome: R-HSA-186763Reactome: R-HSA-1963640Reactome: R-HSA-210993Reactome: R-HSA-2179392Reactome: R-HSA-2424491Reactome: R-HSA-2428933Reactome: R-HSA-2871796Reactome: R-HSA-375165Reactome: R-HSA-3928662Reactome: R-HSA-442982Reactome: R-HSA-5218921Reactome: R-HSA-5621575Reactome: R-HSA-5637810Reactome: R-HSA-5654688Reactome: R-HSA-5654693Reactome: R-HSA-5654699Reactome: R-HSA-5654700Reactome: R-HSA-5654704Reactome: R-HSA-5654706Reactome: R-HSA-5654712Reactome: R-HSA-5654719Reactome: R-HSA-5655253Reactome: R-HSA-5655291Reactome: R-HSA-5655302Reactome: R-HSA-5655332Reactome: R-HSA-5658442Reactome: R-HSA-5673000Reactome: R-HSA-5673001Reactome: R-HSA-5674135Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802953Reactome: R-HSA-6802955Reactome: R-HSA-74751Reactome: R-HSA-8849471Reactome: R-HSA-8851805Reactome: R-HSA-9026519Reactome: R-HSA-9027284Reactome: R-HSA-9028731Reactome: R-HSA-9034864Reactome: R-HSA-9607240Reactome: R-HSA-9634285Reactome: R-HSA-9634635Reactome: R-HSA-9648002Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9665686Reactome: R-HSA-9670439Reactome: R-HSA-9673767Reactome: R-HSA-9673770Reactome: R-HSA-9703465Reactome: R-HSA-9703648Reactome: R-HSA-9753510Reactome: R-HSA-9753512 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideGolgi ApparatusGolgi Apparatus MembraneThe Active Gtp-Bound Form Is Localized Most Strongly To Membranes Than The Inactive Gdp-Bound FormShuttles Between The Plasma Membrane And The Golgi ApparatusIsoform 2: NucleusCytoplasmPerinuclear RegionColocalizes With Rack1 To The Perinuclear Region |
| Alternative Peptide Names | Gtpase Hras proteinH-Ras-1 proteinHa-Ras proteinTransforming Protein P21 proteinC-H-Ras proteinP21ras Cleaved Into - Gtpase Hras - N-Terminally Processed proteinHRAS proteinHRAS1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance