• Western blot analysis of extracts of HeLa cells, using GAPDH antibody as the primary antibody. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) antibody (STJS000854) at 1:5000/1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Goat Anti-Mouse IgG H&L antibody {HRP} (STJS000854)

SKU:
STJS000854-100

Current Stock:
Host: Goat
Applications: WB
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: HRP-conjugated goat anti-NA Mouse IgG H&L secondary antibody. For use in most research applications.
Conjugation: HRP
Formulation: PBS with 0.75% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: ELISA 1:5000-1:10000
WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Mouse IgG
Immunogen Sequence: Email for sequence
Background This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E) , which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5 end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Information sourced from Uniprot.org

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