| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
FYN Blocking Peptide for STJ501111 is synthetically produced from the 240-290 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
FYN |
| Gene ID: |
2534 |
| Uniprot ID: |
FYN_HUMAN |
| Immunogen Region: |
240-290 |
| Specificity: |
This blocking peptide is recommended for use in combination with FYN antibody, STJ501111 |
| Immunogen: |
Synthetic peptide taken within amino acid region 240-290 on human Fyn protein. |
| Post Translational Modifications | Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. Phosphorylated at tyrosine residues, which can be enhanced by NTN1. Palmitoylated. Palmitoylation at Cys-3 and Cys-6, probably by ZDHHC21, regulates subcellular location. |
| Function | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling. |
| Peptide Name | Tyrosine-Protein Kinase FynProto-Oncogene SynProto-Oncogene C-FynSrc-Like KinaseSlkP59-Fyn |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-1227986Reactome: R-HSA-1257604Reactome: R-HSA-1433557Reactome: R-HSA-1433559Reactome: R-HSA-164944Reactome: R-HSA-202733Reactome: R-HSA-2029481Reactome: R-HSA-210990Reactome: R-HSA-2219530Reactome: R-HSA-2424491Reactome: R-HSA-2682334Reactome: R-HSA-2730905Reactome: R-HSA-373753Reactome: R-HSA-375165Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-389513Reactome: R-HSA-3928662Reactome: R-HSA-3928663Reactome: R-HSA-3928664Reactome: R-HSA-3928665Reactome: R-HSA-399954Reactome: R-HSA-399955Reactome: R-HSA-399956Reactome: R-HSA-418885 P06241-1Reactome: R-HSA-4420097Reactome: R-HSA-5621480Reactome: R-HSA-5621575Reactome: R-HSA-5673001Reactome: R-HSA-6811558Reactome: R-HSA-75892Reactome: R-HSA-8866376Reactome: R-HSA-9032500 P06241-1Reactome: R-HSA-9032759 P06241-1Reactome: R-HSA-912631Reactome: R-HSA-9664323Reactome: R-HSA-9664422Reactome: R-HSA-9670439Reactome: R-HSA-9680350Reactome: R-HSA-9706374Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmNucleusCell MembranePerikaryonPresent And Active In Lipid RaftsPalmitoylation Is Crucial For Proper Trafficking |
| Alternative Peptide Names | Tyrosine-Protein Kinase Fyn proteinProto-Oncogene Syn proteinProto-Oncogene C-Fyn proteinSrc-Like Kinase proteinSlk proteinP59-Fyn proteinFYN protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
