| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Ephrin Receptor B3 Blocking Peptide for STJ500938 is synthetically produced from the 100-200 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Ephb3 |
| Gene ID: |
13845 |
| Uniprot ID: |
EPHB3_MOUSE |
| Immunogen Region: |
100-200 |
| Specificity: |
This blocking peptide is recommended for use in combination with Ephrin Receptor B3 antibody, STJ500938 |
| Immunogen: |
Synthetic peptide taken within amino acid region 100-200 on EphBR3 protein. |
| Tissue Specificity | Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk. Expressed by Paneth and progenitor cells in the crypts of the intestinal epithelium (at protein level). Expressed in myogenic progenitor cells. |
| Post Translational Modifications | Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-609 is required for interaction with SH2 domain-containing proteins. Ubiquitinated by RNF186, mainly through 'Lys-48' and 'Lys-63'-linked polyubiquitin chains. |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt. |
| Peptide Name | Ephrin Type-B Receptor 3Developmental Kinase 5Mdk-5Tyrosine-Protein Kinase Receptor Sek-4 |
| Database Links | Reactome: R-MMU-2682334Reactome: -MMU-3928662Reactome: -MMU-3928664Reactome: -MMU-3928665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionDendrite |
| Alternative Peptide Names | Ephrin Type-B Receptor 3 proteinDevelopmental Kinase 5 proteinMdk-5 proteinTyrosine-Protein Kinase Receptor Sek-4 proteinEphb3 proteinEtk2 proteinMdk5 proteinSek4 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
