| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Phospho-ENAH Blocking Peptide for STJ506413 is synthetically produced from the 710-760 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Dilution Range: |
ELISA 1:10, 000IP 1:200WB 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Enah |
| Gene ID: |
13800 |
| Uniprot ID: |
ENAH_MOUSE |
| Immunogen Region: |
710-760 |
| Specificity: |
This blocking peptide is recommended for use in combination with ENAH antibody, STJ506413 |
| Tissue Specificity | Expressed in heart and testis, lower levels in lung, skeletal muscle, kidney, pancreas and brain. Isoform 5 is expressed exclusively in the brain. Isoform 2 is expressed predominantly in brain, testis, ovary and fat. In the brain, isoforms 2 and 5 are expressed at highest levels in the hippocampus, cortex and midbrain, and at lowest levels in the striatum and cerebellum. Isoform 6 is expressed in brain and spleen. |
| Post Translational Modifications | NTN1-induced PKA phosphorylation on Ser-255 directly parallels the formation of filopodial protrusions. |
| Function | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. |
| Peptide Name | Protein Enabled HomologNpc-Derived Proline-Rich Protein 1Ndpp-1 |
| Database Links | Reactome: R-MMU-376176 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionLamellipodiumFilopodiumSynapseCell JunctionFocal AdhesionTargeted To The Leading Edge Of Lamellipodia And Filopodia By Mrl Family MembersColocalizes At Filopodial Tips With A Number Of Other Proteins Including Vinculin And ZyxlinColocalizes With N-Wasp At The Leading EdgeColocalizes With Gphn And Pfn At Synapses |
| Alternative Peptide Names | Protein Enabled Homolog proteinNpc-Derived Proline-Rich Protein 1 proteinNdpp-1 proteinEnah proteinMena proteinNdpp1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
