| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
EHMT1/GLP1 Positive Control for STJ506055 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
EHMT1 |
| Gene ID: |
79813 |
| Uniprot ID: |
EHMT1_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with EHMT1/GLP1 antibody STJ506055. |
| Immunogen: |
Purified protein. |
| Tissue Specificity | Widely expressed. |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with probable chromatin reader BAZ2B. |
| Peptide Name | Histone-Lysine N-Methyltransferase Ehmt1Euchromatic Histone-Lysine N-Methyltransferase 1Eu-Hmtase1G9a-Like Protein 1GlpGlp1Histone H3-K9 Methyltransferase 5H3-K9-Hmtase 5Lysine N-Methyltransferase 1d |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-3214841Reactome: R-HSA-6804760Reactome: R-HSA-8853884Reactome: R-HSA-8953750 |
| Cellular Localisation | NucleusChromosomeAssociates With Euchromatic Regions |
| Alternative Peptide Names | Histone-Lysine N-Methyltransferase Ehmt1 proteinEuchromatic Histone-Lysine N-Methyltransferase 1 proteinEu-Hmtase1 proteinG9a-Like Protein 1 proteinGlp proteinGlp1 proteinHistone H3-K9 Methyltransferase 5 proteinH3-K9-Hmtase 5 proteinLysine N-Methyltransferase 1d proteinEHMT1 proteinEUHMTASE1 proteinGLP proteinKIAA1876 proteinKMT1D protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
