| Host: | E.coli |
| Applications: | SDS-PAGE/Enzyme Activity |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-E.coli Carbonic anhydrase 2/CA2-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Specific activity is > 1, 000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C. |
| Immunogen Region: | 1-220aa |
| Accession Number: | NP_414668 |
| Immunogen: | E.coli |
| Immunogen Sequence: |
| Background | Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O <-> HCO3-+ H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E. coli and purified by conventional chromatography techniques. |
Information sourced from Uniprot.org