| Host: | E.coli |
| Applications: | SDS-PAGE |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-dengue virus 2 Envelope-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% Glycerol, 1mM DTT |
| Concentration: | 0.5 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: | 298-400aa |
| Accession Number: | AAA42954 |
| Immunogen: | Dengue virus 2 |
| Immunogen Sequence: |
| Background | Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. This protein is synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes. Recombinant Dengue virus DENV protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Information sourced from Uniprot.org