CCK4/PTK7 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:500
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Purified Protein tyrosine kinase 7 protein was emulsified in complete adjuvant and used to immunize the rabbits.
Additional Info
Tissue Specificity
Highly expressed in brain and kidney.
Post Translational Modifications
Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated. The activity of MAP3K12 can be regulated through its proteasomal degradation. APOE, through a receptor-mediated mechanism, activates MAP3K12 by preventing its proteasomal degradation.
Function
Part of a non-canonical MAPK signaling pathway. Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1. May be an activator of the JNK/SAPK pathway.
Peptide Name
Mitogen-Activated Protein Kinase Kinase Kinase 12Dual Leucine Zipper Bearing KinaseDlkLeucine-Zipper Protein KinaseZpkMapk-Upstream KinaseMukMixed Lineage Kinase
Cellular Localisation
CytoplasmCell MembraneBehaves Essentially As An Integral Membrane Protein
Alternative Peptide Names
Mitogen-Activated Protein Kinase Kinase Kinase 12 proteinDual Leucine Zipper Bearing Kinase proteinDlk proteinLeucine-Zipper Protein Kinase proteinZpk proteinMapk-Upstream Kinase proteinMuk proteinMixed Lineage Kinase proteinMAP3K12 proteinZPK protein
