| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Aurora B Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:2, 500-1:5, 000 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. Isoform 3: Not expressed in normal liver, high expression in metastatic liver. |
| Post Translational Modifications | The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity. Acetylated at Lys-215 by KAT5 at kinetochores, increasing AURKB activity and promoting accurate chromosome segregation in mitosis. Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. Deubiquitinated by USP35.inhibiting CDH1-mediated degradation of AURKB. |
| Function | Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes. Acts as an inhibitor of CGAS during mitosis: catalyzes phosphorylation of the N-terminus of CGAS during the G2-M transition, blocking CGAS liquid phase separation and activation, and thereby preventing CGAS-induced autoimmunity. Phosphorylates KRT5 during anaphase and telophase. |
| Peptide Name | Aurora Kinase BAurora 1Aurora- And Ipl1-Like Midbody-Associated Protein 1Aim-1Aurora/Ipl1-Related Kinase 2Ark-2Aurora-Related Kinase 2Stk-1Serine/Threonine-Protein Kinase 12Serine/Threonine-Protein Kinase 5Serine/Threonine-Protein Kinase Aurora-B |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-174178Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-4615885Reactome: R-HSA-5663220Reactome: R-HSA-6804756Reactome: R-HSA-68877Reactome: R-HSA-9022692Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreCytoplasmCytoskeletonSpindleMidbodyLocalizes On Chromosome Arms And Inner Centromeres From Prophase Through Metaphase And Then Transferring To The Spindle Midzone And Midbody From Anaphase Through CytokinesisColocalized With Gamma Tubulin In The MidbodyProper Localization Of The ActiveThr-232-Phosphorylated Form During Metaphase May Be Dependent Upon Interaction With SpdycColocalized With Sirt2 During Cytokinesis With The MidbodyLocalization (And Probably Targeting Of The Cpc) To The Inner Centromere Occurs Predominantly In Regions With Overlapping Mitosis-Specific Histone Phosphorylations H3pt3 And H2apt12 |
| Alternative Peptide Names | Aurora Kinase B proteinAurora 1 proteinAurora- And Ipl1-Like Midbody-Associated Protein 1 proteinAim-1 proteinAurora/Ipl1-Related Kinase 2 proteinArk-2 proteinAurora-Related Kinase 2 proteinStk-1 proteinSerine/Threonine-Protein Kinase 12 proteinSerine/Threonine-Protein Kinase 5 proteinSerine/Threonine-Protein Kinase Aurora-B proteinAURKB proteinAIK2 proteinAIM1 proteinAIRK2 proteinARK2 proteinSTK1 proteinSTK12 proteinSTK5 protein |
Information sourced from Uniprot.org
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