| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
ASNA1 Positive Control for STJ500179 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
GET3 |
| Gene ID: |
439 |
| Uniprot ID: |
GET3_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with ASNA1 antibody STJ500179. |
| Tissue Specificity | Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle. |
| Function | ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling. |
| Peptide Name | Atpase Get3Arsenical Pump-Driving AtpaseArsenite-Stimulated AtpaseGuided Entry Of Tail-Anchored Proteins Factor 3 - AtpaseTransmembrane Domain Recognition Complex 40 Kda Atpase SubunitHarsa-IHasna-I |
| Database Links | Reactome: R-HSA-9609523 |
| Cellular Localisation | CytoplasmEndoplasmic ReticulumNucleusNucleolus |
| Alternative Peptide Names | Atpase Get3 proteinArsenical Pump-Driving Atpase proteinArsenite-Stimulated Atpase proteinGuided Entry Of Tail-Anchored Proteins Factor 3 - Atpase proteinTransmembrane Domain Recognition Complex 40 Kda Atpase Subunit proteinHarsa-I proteinHasna-I proteinGET3 proteinARSA proteinASNA1 proteinTRC40 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
