| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ZNF598 (120-420) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ZNF598 |
| Gene ID: |
90850 |
| Uniprot ID: |
ZN598_HUMAN |
| Immunogen Region: |
120-420 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 120-420 of human ZNF598 (NP_835461.2). |
| Immunogen Sequence: |
PRCPELPPFSLFGDLEQHMR RQHELFCCRLCLQHLQIFTY ERKWYSRKDLARHRMQGDPD DTSHRGHPLCKFCDERYLDN DELLKHLRRDHYFCHFCDSD GAQDYYSDYAYLREHFREKH FLCEEGRCSTEQFTHAFRTE IDLKAHRTACHSRSRAEARQ NRHIDLQFSYAPRHSRRNEG VVGGEDYEEVDRYSRQGRVA RAGTRGAQQSRRGSWRYKRE EEDREVAAAVRASVAAQQQ |
| Function | E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, leading to degradation of nascent peptide chains. ZNF598 is activated when ribosomes are stalled within an mRNA following translation of prematurely polyadenylated mRNAs. Acts as a ribosome collision sensor: specifically recognizes and binds collided di-ribosome, which arises when a trailing ribosome encounters a slower leading ribosome, leading to terminally arrest translation. Following binding to colliding ribosomes, mediates monoubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS3/uS3, and 'Lys-63'-linked polyubiquitination of RPS20/uS10. Polyubiquitination of RPS20/uS10 promotes recruitment of the RQT (ribosome quality control trigger) complex, which drives the disassembly of stalled ribosomes, followed by degradation of nascent peptides. E3 ubiquitin-protein ligase activity is dependent on the E2 ubiquitin-conjugating enzyme UBE2D3. Also acts as an adapter that recruits the 4EHP-GYF2 complex to mRNAs. Independently of its role in RQC, may also act as a negative regulator of interferon-stimulated gene (ISG) expression. (Microbial infection) Required for poxvirus protein synthesis by mediating ubiquitination of RPS10/eS10 and RPS20/uS10. Poxvirus encoding mRNAs contain unusual 5' poly(A) leaders and ZNF598 is required for their translational efficiency, possibly via its ability to suppress readthrough or sliding on shorter poly(A) tracts. |
| Protein Name | E3 Ubiquitin-Protein Ligase Znf598Zinc Finger Protein 598 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Znf598 antibodyAnti-Zinc Finger Protein 598 antibodyAnti-ZNF598 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
