• Western blot analysis of various lysates using ZDHHC8 Rabbit polyclonal antibody (STJ119474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ZDHHC8 antibody (240-380) (STJ119474)

SKU:
STJ119474

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZDHHC8 (240-380) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZDHHC8
Gene ID: 29801
Uniprot ID: ZDHC8_HUMAN
Immunogen Region: 240-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human ZDHHC8 (NP_037505.1).
Immunogen Sequence: VEHVLCSPLAPRYVVEPPRL PLAVSLKPPFLRPELLDRAA PLKVKLSDNGLKAGLGRSKS KGSLDRLDEKPLDLGPPLPP KIEAGTFSSDLQTPRPGSAE SALSVQRTSPPTPAMYKFRP AFPTGPKVPFCGPGEQVPGP D
Tissue Specificity Widely expressed.
Function Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and therefore functions in several unrelated biological processes (Probable). Through the palmitoylation of ABCA1 regulates the localization of the transporter to the plasma membrane and thereby regulates its function in cholesterol and phospholipid efflux (Probable). Could also pamitoylate the D(2) dopamine receptor DRD2 and regulate its stability and localization to the plasma membrane (Probable). Could also play a role in glutamatergic transmission. (Microbial infection) Able to palmitoylate SARS coronavirus-2/SARS-CoV-2 spike protein following its synthesis in the endoplasmic reticulum (ER). In the infected cell, promotes spike biogenesis by protecting it from premature ER degradation, increases half-life and controls the lipid organization of its immediate membrane environment. Once the virus has formed, spike palmitoylation controls fusion with the target cell.
Protein Name Palmitoyltransferase Zdhhc8
Zinc Finger Dhhc Domain-Containing Protein 8
Dhhc-8
Zinc Finger Protein 378
Database Links Reactome: R-HSA-8963896
Reactome: R-HSA-9694548
Cellular Localisation Golgi Apparatus Membrane
Multi-Pass Membrane Protein
Mitochondrion Membrane
Alternative Antibody Names Anti-Palmitoyltransferase Zdhhc8 antibody
Anti-Zinc Finger Dhhc Domain-Containing Protein 8 antibody
Anti-Dhhc-8 antibody
Anti-Zinc Finger Protein 378 antibody
Anti-ZDHHC8 antibody
Anti-KIAA1292 antibody
Anti-ZDHHCL1 antibody
Anti-ZNF378 antibody

Information sourced from Uniprot.org

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