Anti-ZAR1 antibody (Internal) (STJ96298)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the Internal region of human ZAR1.
General Information
| Short Description | Rabbit polyclonal anti-Zygote arrest protein 1 (Internal) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC-P 100-300WB 1:500-1:2000ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ZAR1 |
| Gene ID | 326340 |
| Uniprot ID | ZAR1_HUMAN |
| Immunogen | Synthesized peptide derived from the Internal region of human ZAR1. |
| Immunogen Region | Internal |
| Specificity | ZAR1 Polyclonal Antibody detects endogenous levels of ZAR1 protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated and degradaded by the proteasome during oocyte meiotic maturation, leading to MARDO (mitochondria-associated ribonucleoprotein domain) membraneless compartment dissolution. |
| Function | mRNA-binding protein that mediates formation of MARDO (mitochondria-associated ribonucleoprotein domain), a membraneless compartment that stores maternal mRNAs in oocytes. MARDO assembly around mitochondria is directed by an increase in mitochondrial membrane potential during oocyte growth. Promotes formation of MARDO phase-separated membraneless compartment by undergoing liquid-liquid phase separation upon binding to maternal mRNAs. Binds to the 3'-UTR of maternal mRNAs. Maternal mRNAs stored in the MARDO are translationally repressed. Essential for female fertility and oocyte-to-embryo transition by coordinating maternal mRNA storage, translation and degradation. |
| Protein Name | Zygote Arrest Protein 1 |
| Database Links | |
| Cellular Localisation | CytoplasmCytoplasmic Ribonucleoprotein GranuleSpecifically Localizes To Mardo (Mitochondria-Associated Ribonucleoprotein Domain)A Mitochondria-Associated Membraneless Compartment That Stores Mrnas In Oocytes |
| Alternative Antibody Names | Anti-Zygote Arrest Protein 1 antibodyAnti-ZAR1 antibody |
Information sourced from Uniprot.org