• Immunoprecipitation analysis of Jurkat cell lysates (ZAP-70 positive expression cell line) and Ramos cell lysates (ZAP-70 negative expression cell line) using ZAP-70 mouse mAb.
  • Western blot detection of ZAP-70 in CEM and Jurkat cell lysates, negative in the Ramos cell lysates using ZAP-70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Anti-ZAP70 antibody [4B2-C1-H3] (STJ99214)

SKU:
STJ99214

Current Stock:
Host: Mouse
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Tyrosine-protein kinase ZAP-70 is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: 4B2-C1-H3
Conjugation: Unconjugated
Isotype: IgG2b
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZAP70
Gene ID: 7535
Uniprot ID: ZAP70_HUMAN
Specificity: This antibody detects endogenous levels of ZAP-70 and does not cross-react with related proteins.
Immunogen: Purified recombinant human ZAP-70 protein fragments expressed in E.coli.
Post Translational Modifications Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22. Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.
Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
Protein Name Tyrosine-Protein Kinase Zap-70
70 Kda Zeta-Chain Associated Protein
Syk-Related Tyrosine Kinase
Database Links Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-9013407
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
In Quiescent T-Lymphocytes
It Is Cytoplasmic
Upon Tcr Activation
It Is Recruited At The Plasma Membrane By Interacting With Cd247/Cd3z
Colocalizes Together With Rhoh In The Immunological Synapse
Rhoh Is Required For Its Proper Localization To The Cell Membrane And Cytoskeleton Fractions In The Thymocytes
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Zap-70 antibody
Anti-70 Kda Zeta-Chain Associated Protein antibody
Anti-Syk-Related Tyrosine Kinase antibody
Anti-ZAP70 antibody
Anti-SRK antibody

Information sourced from Uniprot.org

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