| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ZAK (1-330) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:20-1:50 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAP3K20 |
| Gene ID: |
51776 |
| Uniprot ID: |
M3K20_HUMAN |
| Immunogen Region: |
1-330 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ZAK (NP_598407.1). |
| Immunogen Sequence: |
MSSLGASFVQIKFDDLQFFE NCGGGSFGSVYRAKWISQDK EVAVKKLLKIEKEAEILSVL SHRNIIQFYGVILEPPNYGI VTEYASLGSLYDYINSNRSE EMDMDHIMTWATDVAKGMHY LHMEAPVKVIHRDLKSRNVV IAADGVLKICDFGASRFHNH TTHMSLVGTFPWMAPEVIQS LPVSETCDTYSYGVVLWEML TREVPFKGLEGLQVAWLVVE KNERLTIPSSCPRSFAELL |
| Tissue Specificity | Ubiquitously expressed. Isoform ZAKbeta is the predominant form in all tissues examined, except for liver, in which isoform ZAKalpha is more highly expressed. |
| Post Translational Modifications | Activated by phosphorylation by PKN1, followed by autophosphorylation on Thr-161 and Ser-165. Autophosphorylation in response to ribotoxic stress promotes dissociation from colliding ribosomes and activation. |
| Function | Stress-activated component of a protein kinase signal transduction cascade that promotes programmed cell death in response to various stress, such as ribosomal stress, osmotic shock and ionizing radiation. Acts by catalyzing phosphorylation of MAP kinase kinases, leading to activation of the JNK (MAPK8/JNK1, MAPK9/JNK2 and/or MAPK10/JNK3) and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways. Activates JNK through phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7, and MAP kinase p38 gamma (MAPK12) via phosphorylation of MAP2K3/MKK3 and MAP2K6/MKK6. Involved in stress associated with adrenergic stimulation: contributes to cardiac decompensation during periods of acute cardiac stress. May be involved in regulation of S and G2 cell cycle checkpoint by mediating phosphorylation of CHEK2. Isoform ZAKalpha: Key component of the stress-activated protein kinase signaling cascade in response to ribotoxic stress or UV-B irradiation. Acts as the proximal sensor of ribosome collisions during the ribotoxic stress response (RSR): directly binds to the ribosome by inserting its flexible C-terminus into the ribosomal intersubunit space, thereby acting as a sentinel for colliding ribosomes. Upon ribosome collisions, activates either the stress-activated protein kinase signal transduction cascade or the integrated stress response (ISR), leading to programmed cell death or cell survival, respectively. Dangerous levels of ribosome collisions trigger the autophosphorylation and activation of MAP3K20, which dissociates from colliding ribosomes and phosphorylates MAP kinase kinases, leading to activation of the JNK and MAP kinase p38 pathways that promote programmed cell death. Less dangerous levels of ribosome collisions trigger the integrated stress response (ISR): MAP3K20 activates EIF2AK4/GCN2 independently of its protein-kinase activity, promoting EIF2AK4/GCN2-mediated phosphorylation of EIF2S1/eIF-2-alpha. Also part of the stress-activated protein kinase signaling cascade triggering the NLRP1 inflammasome in response to UV-B irradiation: ribosome collisions activate MAP3K20, which directly phosphorylates NLRP1, leading to activation of the NLRP1 inflammasome and subsequent pyroptosis. NLRP1 is also phosphorylated by MAP kinase p38 downstream of MAP3K20. Also acts as a histone kinase by phosphorylating histone H3 at 'Ser-28' (H3S28ph). Isoform ZAKbeta: Isoform that lacks the C-terminal region that mediates ribosome-binding: does not act as a sensor of ribosome collisions in response to ribotoxic stress. May act as an antagonist of isoform ZAKalpha: interacts with isoform ZAKalpha, leading to decrease the expression of isoform ZAKalpha. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 20Human Cervical Cancer Suppressor Gene 4 ProteinHccs-4Leucine Zipper- And Sterile Alpha Motif-Containing KinaseMlk-Like Mitogen-Activated Protein Triple KinaseMitogen-Activated Protein Kinase Kinase Kinase MltMixed Lineage Kinase 7Mixed Lineage Kinase-Related KinaseMlk-Related KinaseMrkSterile Alpha Motif- And Leucine Zipper-Containing Kinase Azk |
| Cellular Localisation | CytoplasmNucleusTranslocates To The Nucleus Upon Ultraviolet B Irradiation |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 20 antibodyAnti-Human Cervical Cancer Suppressor Gene 4 Protein antibodyAnti-Hccs-4 antibodyAnti-Leucine Zipper- And Sterile Alpha Motif-Containing Kinase antibodyAnti-Mlk-Like Mitogen-Activated Protein Triple Kinase antibodyAnti-Mitogen-Activated Protein Kinase Kinase Kinase Mlt antibodyAnti-Mixed Lineage Kinase 7 antibodyAnti-Mixed Lineage Kinase-Related Kinase antibodyAnti-Mlk-Related Kinase antibodyAnti-Mrk antibodyAnti-Sterile Alpha Motif- And Leucine Zipper-Containing Kinase Azk antibodyAnti-MAP3K20 antibodyAnti-MLK7 antibodyAnti-MLTK antibodyAnti-ZAK antibodyAnti-HCCS4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
