Anti-YWHAE antibody (206-255 aa) (STJ91370)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human 14-3-3 epsilon at the amino acid range 206-255
General Information
| Short Description | Rabbit polyclonal anti-14-3-3 protein epsilon (206-255 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | YWHAE |
| Gene ID | 7531 |
| Uniprot ID | 1433E_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human 14-3-3 epsilon at the amino acid range 206-255 |
| Immunogen Region | 206-255 aa |
| Specificity | 14-3-3 Epsilon Polyclonal Antibody detects endogenous levels of 14-3-3 Epsilon protein. |
Additional Info
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm. Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI. Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection. Plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm to be degraded by ubiquitination. |
| Protein Name | 14-3-3 Protein Epsilon14-3-3e |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-1445148Reactome: R-HSA-2028269Reactome: R-HSA-205025Reactome: R-HSA-2565942Reactome: R-HSA-3371453Reactome: R-HSA-3371511Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-5625740Reactome: R-HSA-5628897Reactome: R-HSA-75035Reactome: R-HSA-8854518Reactome: R-HSA-8862803Reactome: R-HSA-8876198Reactome: R-HSA-9735871Reactome: R-HSA-9755779Reactome: R-HSA-9856649 |
| Cellular Localisation | NucleusCytoplasmMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-14-3-3 Protein Epsilon antibodyAnti-14-3-3e antibodyAnti-YWHAE antibody |
Information sourced from Uniprot.org