| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-YES1 (1-250) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
YES1 |
| Gene ID: |
7525 |
| Uniprot ID: |
YES_HUMAN |
| Immunogen Region: |
1-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human YES1 (NP_005424.1). |
| Immunogen Sequence: |
MGCIKSKENKSPAIKYRPEN TPEPVSTSVSHYGAEPTTVS PCPSSSAKGTAVNFSSLSMT PFGGSSGVTPFGGASSSFSV VPSSYPAGLTGGVTIFVALY DYEARTTEDLSFKKGERFQI INNTEGDWWEARSIATGKNG YIPSNYVAPADSIQAEEWYF GKMGRKDAERLLLNPGNQRG IFLVRESETTKGAYSLSIRD WDEIRGDNVKHYKIRKLDNG GYYITTRAQFDTLQKLVKH |
| Tissue Specificity | Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis. |
| Post Translational Modifications | Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-426 maintains enzyme activity by blocking CSK-mediated inhibition. Palmitoylation at Cys-3 promotes membrane localization. |
| Function | Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity. |
| Protein Name | Tyrosine-Protein Kinase YesProto-Oncogene C-YesP61-Yes |
| Database Links | Reactome: R-HSA-1227986Reactome: R-HSA-1433557Reactome: R-HSA-1433559Reactome: R-HSA-2029481Reactome: R-HSA-210990Reactome: R-HSA-2682334Reactome: R-HSA-389356Reactome: R-HSA-389513Reactome: R-HSA-3928662Reactome: R-HSA-3928663Reactome: R-HSA-3928665Reactome: R-HSA-8940973Reactome: R-HSA-912631Reactome: R-HSA-9664323Reactome: R-HSA-9664422Reactome: R-HSA-9670439Reactome: R-HSA-9680350 |
| Cellular Localisation | Cell MembraneCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCytosolNewly Synthesized Protein Initially Accumulates In The Golgi Region And Traffics To The Plasma Membrane Through The Exocytic Pathway |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Yes antibodyAnti-Proto-Oncogene C-Yes antibodyAnti-P61-Yes antibodyAnti-YES1 antibodyAnti-YES antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
