| Host: | Mouse |
| Applications: | WB/ICC/IP |
| Reactivity: | Human/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-X-ray repair cross-complementing protein 6 is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 7B1-E12-G4 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000 ICC 1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | XRCC6 |
| Gene ID: | 2547 |
| Uniprot ID: | XRCC6_HUMAN |
| Specificity: | This antibody detects endogenous levels of Ku70 and does not cross-react with related proteins. |
| Immunogen: | Purified recombinant human Ku70 protein fragments expressed in E.coli. |
| Function | Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. |
| Protein Name | X-Ray Repair Cross-Complementing Protein 6 5'-Deoxyribose-5-Phosphate Lyase Ku70 5'-Drp Lyase Ku70 70 Kda Subunit Of Ku Antigen Atp-Dependent Dna Helicase 2 Subunit 1 Atp-Dependent Dna Helicase Ii 70 Kda Subunit Ctc Box-Binding Factor 75 Kda Subunit Ctc75 Ctcbf Dna Repair Protein Xrcc6 Lupus Ku Autoantigen Protein P70 Ku70 Thyroid-Lupus Autoantigen Tlaa X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6 |
| Database Links | Reactome: R-HSA-164843 Reactome: R-HSA-1834949 Reactome: R-HSA-3270619 Reactome: R-HSA-5693571 Reactome: R-HSA-6798695 |
| Cellular Localisation | Nucleus Chromosome |
| Alternative Antibody Names | Anti-X-Ray Repair Cross-Complementing Protein 6 antibody Anti-5'-Deoxyribose-5-Phosphate Lyase Ku70 antibody Anti-5'-Drp Lyase Ku70 antibody Anti-70 Kda Subunit Of Ku Antigen antibody Anti-Atp-Dependent Dna Helicase 2 Subunit 1 antibody Anti-Atp-Dependent Dna Helicase Ii 70 Kda Subunit antibody Anti-Ctc Box-Binding Factor 75 Kda Subunit antibody Anti-Ctc75 antibody Anti-Ctcbf antibody Anti-Dna Repair Protein Xrcc6 antibody Anti-Lupus Ku Autoantigen Protein P70 antibody Anti-Ku70 antibody Anti-Thyroid-Lupus Autoantigen antibody Anti-Tlaa antibody Anti-X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6 antibody Anti-XRCC6 antibody Anti-G22P1 antibody |
Information sourced from Uniprot.org