| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-DNA repair protein XRCC1 (517-566 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | XRCC1 |
| Gene ID: | 7515 |
| Uniprot ID: | XRCC1_HUMAN |
| Immunogen Region: | 517-566 aa |
| Specificity: | XRCC1 Polyclonal Antibody detects endogenous levels of XRCC1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human XRCC1 at the amino acid range 517-566 |
| Post Translational Modifications | Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated. |
| Function | Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity. |
| Protein Name | Dna Repair Protein Xrcc1X-Ray Repair Cross-Complementing Protein 1 |
| Database Links | Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5685939Reactome: R-HSA-5696397Reactome: R-HSA-6782210 |
| Cellular Localisation | NucleusChromosomeMoves From The Nucleoli To The Global Nuclear Chromatin Upon Dna DamageRecruited To Dna Damage Sites Fowwing Interaction With Poly-Adp-Ribose Chains |
| Alternative Antibody Names | Anti-Dna Repair Protein Xrcc1 antibodyAnti-X-Ray Repair Cross-Complementing Protein 1 antibodyAnti-XRCC1 antibody |
Information sourced from Uniprot.org
