• Western blot detection of XRCC1 in Raji, K562, Hela, Jurkat and THP-1 cell lysates using XRCC1 mouse mAb (1:1000 diluted).Predicted band size:82KDa.Observed band size:82KDa.

Anti-XRCC1 antibody [3F6-G7-F7] (STJ99239)

SKU:
STJ99239

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-DNA repair protein XRCC1 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 3F6-G7-F7
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XRCC1
Gene ID: 7515
Uniprot ID: XRCC1_HUMAN
Specificity: This antibody detects endogenous levels of XRCC1 and does not cross-react with related proteins.
Immunogen: Purified recombinant human XRCC1 protein fragments expressed in E.coli.
Post Translational Modifications Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated.
Function Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity.
Protein Name Dna Repair Protein Xrcc1
X-Ray Repair Cross-Complementing Protein 1
Database Links Reactome: R-HSA-110381
Reactome: R-HSA-5649702
Reactome: R-HSA-5685939
Reactome: R-HSA-5696397
Reactome: R-HSA-6782210
Cellular Localisation Nucleus
Chromosome
Moves From The Nucleoli To The Global Nuclear Chromatin Upon Dna Damage
Recruited To Dna Damage Sites Fowwing Interaction With Poly-Adp-Ribose Chains
Alternative Antibody Names Anti-Dna Repair Protein Xrcc1 antibody
Anti-X-Ray Repair Cross-Complementing Protein 1 antibody
Anti-XRCC1 antibody

Information sourced from Uniprot.org

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