Western blot analysis of extracts of various cell lines, using WTAP antibody (STJ116896) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-WTAP antibody (212-355) (STJ116896)

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STJ116896
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WTAP
Gene ID: 9589
Uniprot ID: FL2D_HUMAN
Immunogen Region: 212-355
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 212-355 of human WTAP (NP_004897.2).
Immunogen Sequence: DEEVEGMQSTILVLQQQLKE TRQQLAQYQQQQSQASAPST SRTTASEPVEQSEATSKDCS RLTNGPSNGSSSRQRTSGSG FHREGNTTEDDFPSSPGNGN KSSNSSEERTGRGGSGYVNQ LSAGYESVDSPTGSENSLTH QSND
Tissue Specificity Ubiquitously expressed.
Function Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.
Protein Name Pre-Mrna-Splicing Regulator Wtap
Female-Lethal(2d Homolog
Hfl(2d
Wt1-Associated Protein
Wilms Tumor 1-Associating Protein
Database Links Reactome: R-HSA-72203
Cellular Localisation Nucleus Speckle
Nucleus
Nucleoplasm
Cytoplasm
Mainly Nuclear With Some Fraction Located In The Cytoplasm
Zc3h13 Is Required To Anchor Component Of The Macom Subcomplex
Such As Virma
In The Nucleus
Alternative Antibody Names Anti-Pre-Mrna-Splicing Regulator Wtap antibody
Anti-Female-Lethal(2d Homolog antibody
Anti-Hfl(2d antibody
Anti-Wt1-Associated Protein antibody
Anti-Wilms Tumor 1-Associating Protein antibody
Anti-WTAP antibody
Anti-KIAA0105 antibody

Information sourced from Uniprot.org

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