• K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-WT1 (ABT-WT1) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 55kDaObserved band size: 55kDa
  • Human endometrial adenocarcinoma tissue was stained with Anti-Wilms' Tumor 1 (WT1) (ABT-WT1) Antibody
  • Human kidney tissue was stained with Anti-Wilms' Tumor 1 (WT1) (ABT-WT1) Antibody
  • Human ovarian serous adenocarcinoma tissue was stained with Anti-Wilms' Tumor 1 (WT1) (ABT-WT1) Antibody

Anti-WT1 antibody (350-449 aa) [ABT-WT1] (STJ196897)

SKU:
STJ196897

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Wilms tumor protein (350-449 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT-WT1
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 500-2000
IHC-P 1:100-500
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WT1
Gene ID: 7490
Uniprot ID: WT1_HUMAN
Immunogen Region: 350-449 aa
Specificity: This antibody detects endogenous levels of human Wilms NA Tumor 1 (WT1). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
Immunogen: Synthesized peptide derived from the human Wilms NA Tumor 1 (WT1) at the amino acid range 350-449
Function Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
Protein Name Wilms Tumor Protein
Wt33
Database Links Reactome: R-HSA-9690406
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Isoforms Lacking The Kts Motif Have A Diffuse Nuclear Location
Shuttles Between Nucleus And Cytoplasm
Isoform 1: Nucleus Speckle
Isoform 4: Nucleus
Nucleoplasm
Alternative Antibody Names Anti-Wilms Tumor Protein antibody
Anti-Wt33 antibody
Anti-WT1 antibody

Information sourced from Uniprot.org

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