| Host: | Mouse |
| Applications: | IHC/WB/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Wilms tumor protein (350-449 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications. |
| Clonality : | Monoclonal |
| Clone ID : | ABT-WT1 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2bk |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range: | WB 500-2000IHC-P 1:100-500IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | WT1 |
| Gene ID: | 7490 |
| Uniprot ID: | WT1_HUMAN |
| Immunogen Region: | 350-449 aa |
| Specificity: | This antibody detects endogenous levels of human Wilms Tumor 1 (WT1). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section |
| Immunogen: | Synthesized peptide derived from the human Wilms Tumor 1 (WT1) at the amino acid range 350-449 |
| Function | Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. |
| Protein Name | Wilms Tumor ProteinWt33 |
| Database Links | Reactome: R-HSA-9690406Reactome: R-HSA-9831926 |
| Cellular Localisation | NucleusNucleolusCytoplasmIsoforms Lacking The Kts Motif Have A Diffuse Nuclear LocationShuttles Between Nucleus And CytoplasmIsoform 1: Nucleus SpeckleIsoform 4: NucleusNucleoplasm |
| Alternative Antibody Names | Anti-Wilms Tumor Protein antibodyAnti-Wt33 antibodyAnti-WT1 antibody |
Information sourced from Uniprot.org