Anti-WT1 antibody (1-302) (STJ118729)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal WT1 (1-302) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WT1 |
| Gene ID | 7490 |
| Uniprot ID | WT1_HUMAN |
| Immunogen Region | 1-302 |
| Immunogen Sequence | MEKGYSTVTFDGTPSYGHTP SHHAAQFPNHSFKHEDPMGQ QGSLGEQQYSVPPPVYGCHT PTDSCTGSQALLLRTPYSSD NLYQMTSQLECMTWNQMNLG ATLKGVAAGSSSSVKWTEGQ SNHSTGYESDNHTTPILCGA QYRIHTHGVFRGIQDVRRVP GVAPTLVRSASETSEKRPFM CAYPGCNKRYFKLSHLQMHS RKHTGEKPYQCDFKDCERRF SRSDQLKRHQRRHTGVKPF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human WT1 (NP_001185480.1). |
Additional Info
| Tissue Specificity | Expressed in the kidney and a subset of hematopoietic cells. |
| Function | Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. |
| Protein Name | Wilms Tumor ProteinWt33 |
| Database Links | Reactome: R-HSA-9690406Reactome: R-HSA-9831926 |
| Cellular Localisation | NucleusNucleolusCytoplasmIsoforms Lacking The Kts Motif Have A Diffuse Nuclear LocationShuttles Between Nucleus And CytoplasmIsoform 1: Nucleus SpeckleIsoform 4: NucleusNucleoplasm |
| Alternative Antibody Names | Anti-Wilms Tumor Protein antibodyAnti-Wt33 antibodyAnti-WT1 antibody |
Information sourced from Uniprot.org