• Western blot analysis of extracts of various cell lines, using WNK3 antibody (STJ27544). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
  • Immunohistochemistry of paraffin-embedded human liver cancer using WNK3 antibody (STJ27544) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human appendix using WNK3 antibody (STJ27544) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spleen using WNK3 antibody (STJ27544) at dilution of 1:100 (40x lens).

Anti-WNK3 antibody (STJ27544)

SKU:
STJ27544

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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: Recombinant protein of rat WNK3
Background Predicted to enable chloride channel inhibitor activity; potassium channel inhibitor activity; and protein serine/threonine kinase activity. Predicted to be involved in several processes, including negative regulation of pancreatic juice secretion; positive regulation of peptidyl-threonine phosphorylation; and regulation of metal ion transport. Predicted to act upstream of or within maintenance of blood-brain barrier. Predicted to be located in adherens junction and bicellular tight junction. Predicted to be active in cytosol. Orthologous to human WNK3 (WNK lysine deficient protein kinase 3).

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance