• Western blot analysis of extracts of mouse ovary, using WISP2 antibody (STJ29592) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using WISP2 rabbit polyclonal antibody (STJ29592) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using WISP2 rabbit polyclonal antibody (STJ29592) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using WISP2 rabbit polyclonal antibody (STJ29592) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using WISP2 rabbit polyclonal antibody (STJ29592) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-WISP2 antibody (164-250) (STJ29592)

SKU:
STJ29592

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WISP2 (164-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCN5
Gene ID: 8839
Uniprot ID: CCN5_HUMAN
Immunogen Region: 164-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 164-250 of human WISP2 (NP_003872.1).
Immunogen Sequence: GQGGGLGTQPLPAQGPQFSG LVSSLPPGVPCPEWSTAWGP CSTTCGLGMATRVSNQNRFC RLETQRRLCLSRPCPPSRGR SPQNSAF
Tissue Specificity Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
Function May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Protein Name Ccn Family Member 5
Connective Tissue Growth Factor-Like Protein
Ctgf-L
Connective Tissue Growth Factor-Related Protein 58
Wnt1-Inducible-Signaling Pathway Protein 2
Wisp-2
Cellular Localisation Secreted
Alternative Antibody Names Anti-Ccn Family Member 5 antibody
Anti-Connective Tissue Growth Factor-Like Protein antibody
Anti-Ctgf-L antibody
Anti-Connective Tissue Growth Factor-Related Protein 58 antibody
Anti-Wnt1-Inducible-Signaling Pathway Protein 2 antibody
Anti-Wisp-2 antibody
Anti-CCN5 antibody
Anti-CT58 antibody
Anti-CTGFL antibody
Anti-WISP2 antibody
Anti-UNQ228 antibody
Anti-PRO261 antibody

Information sourced from Uniprot.org

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